PVA和PEG预处理对大豆种子在低温吸胀过程中胚根线粒体发育和超微结构的影响

电镜观察发现,大豆种子在刚开始萌发时胚根细胞中未能见到线粒体,线粒体是在种子萌发过程中逐渐出现的,由原质体再分化发育而成。对照胚根细胞内原质体在低温吸张过程中明显膨胀,在回温后胚根细胞中原质体仍不能发育成线粒体,甚至网状膜结构破坏,呈空泡化;经聚乙烯醇(PVA)和聚乙二醇(PEG 6000)预处理的大豆种子在同样条件下线粒体能继续发育,在回温后预处理胚根细胞中线粒体发育良好,具有明显的双层膜和管状嵴的结构。这些结果表明,在低温吸胀过程中原质体能够继续再分化发育成线粒体是提高大豆种子活力和抗冷力的重要原因。     

Structure and sequence of the human α- -iduronidase gene

In humans, a deficiency of the lysosomal hydrolase α- -iduronidase (IDUA; EC 3.2.1.76) results in the lysosomal storage of the glycosaminoglycans heparan sulfate and dermatan sulfate, thereby causing the lysosomal storage disorder mucopolysaccharidosis type I. The gene for IDUA is split into 14 exons spanning approximately 19 kb. We report the sequence of two noncontiguous segments of the IDUA gene, one 1.8-kb segment containing exons 1 and 2 and surrounding sequences and a second segment of 4.5 kb containing the last 12 exons. The potential promoter for IDUA has only GC box type consensus sequences consistent with a housekeeping promoter and is bounded by an Alu repeat sequence. The first two exons of IDUA are separated by an intron of 566 bp, then there is a large intron of approximately 13 kb, and the last 12 exons are clustered within 4.5 kb. No consensus polyadenylation signal was found in the 3′ untranslated region, although two variant polyadenylation signals are proposed.     

Monoclonal Antibodies against Apple 1-Aminocyclopropane-l-Carboxylate Synthase

1-Aminocyclopropane-l-carboxylate (ACC) synthase from applefruits was purified over 5,000-fold by conventional column chromatography.By immunizing mice with this partially purified enzyme preparation,8 hybridoma lines producing monoclonal antibodies against appleACC synthase were isolated. While all 8 clones immunoprecipitatednative ACC synthase, only two clones recognized the putative(48 kDa) ACC synthase on Western blots. When a partially purifiedACC synthase preparation was incubated with S-adenosyl-L-[carboxyl-¹⁴C]methionine(AdoMet), only one radioactive protein of 48 kDa was detectedon sodium dodecyl sulfate-poly-acrylamide gel electrophoresis.This radioactive protein was specifically immunoprecipitatedby the monoclonal antibodies, indicating that apple ACC synthaseis specifically radiolabeled by its substrate AdoMet, as istomato ACC synthase. Thus, the monoclonal antibodies recognizedboth native and AdoMet-inactivated forms of ACC synthase. Whilethese antibodies failed to im-munoprecipitate ACC synthase isolatedfrom ripe tomato fruits, ripe avocado fruits or auxin-treatedmungbean hypocotyls, they were effective in immunoprecipitatingthe enzyme isolated from ripe pear fruits. (Received August 11, 1990; Accepted October 17, 1990)     

Technique for repeated collection of cerebrospinal fluid from cisterna magna of anesthetized strain 13 guinea pigs

To study biochemical changes in cerebrospinal fluid (CSF), we developed a reliable technique for repeated collection of CSF in anesthetized strain 13 guinea pigs. The animal's head was mounted in a stereotaxic instrument with ventral tilt at 30 degrees, and cisternal puncture was made with an L-shaped, 23-gauge needle through the shaved skin. Clear CSF was collected in a 1-ml syringe surrounded by crushed ice. Each collection procedure lasted for 3 min, and three consecutive collections produced about 0.2 ml of CSF. Sampling was repeated at 3-hr intervals. With intravenous saline infusion (10 ml/kg.hr), a total volume of 0.6-1.0 ml of CSF was collected over 6 to 12 hr. Animals maintained a mean blood pressure, heart rate, and minute volume, with few changes during CSF sampling for the entire collection.     

羊草草原枯枝落叶分解的研究——主要优势植物的分解速率和损失率

分解速率和损失率从不同侧面反映了枯枝落叶分解动态,羊草草原主要优势植物,羊草（Leymus chinensis),拂子茅（Calamagrostis epigejos),减蓬（Suaeda glauca),碱茅（Puccinellia tenuiflora),五脉山黎豆（Lathyrus quinqueneruivs),碱蒿（Artemisia anethifolia)分解速率的季节变化动态近似倒“V”字型,损失率的季节变化呈S型,反了枯枝落叶的失重情况,枯枝落叶的化学组成成分是造成不同种植物间分解差异的主要原因,特别是C／N比与分解快慢有密切关系,分解初期,枯枝落叶的损失符合指数衰减模型,枯枝落叶损失95％所需时间,羊草群落约为8．8a,杂类草群落约为9．7a,碱茅群落约为7．1a,碱蓬群落约为4．7a。     

黄河鼠兔Ochotona huangensis(Matschie,1907)的分类研究

黄河鼠兔(Ochotona huangensis)由Matschie(1907)发表,长期以来国内外学者意见纷纭,有人将它归入藏鼠兔(O.thibetana),有的则作为达乌尔鼠兔(O.daurica)的同物异名。经查对地模及邻近地模产地的标本,并与其相似种进行了仔细的对比,认为黄河鼠兔不同于藏鼠兔、甘肃鼠兔、达乌尔鼠兔,而是一有效物种。     

努布拉鼠兔(Ochotona nubrica Thomas,1922)的分类订正

努布拉鼠兔(Ochotona nubrica)的分类地位迄今未能得到合理解决,曾被列为草原鼠兔(O.pusilla)、灰鼠兔(O.roylei)或藏鼠兔(O.thibetana)的同物异名。作者根据原始文献、地模标本及邻近地模产地的标本与有关的鼠兔种类进行对比研究,证实了努布拉鼠兔既不同于藏鼠兔,也不同于灰鼠兔,而是一个有效物种。     

9种针阔叶幼树的蒸腾速率、叶水势与环境因子关系的研究

应用QK-1气孔仪和压力室测定了樟子松、青杨等9种针阔叶幼树的蒸腾速率与其叶水势及与环境因子的关系。采用多元线性回归和逐步回归对观测值的统计分析结果表明:在土壤供水良好的条件下,其蒸腾速率主要受气象因子的影响,与其叶水势的相关性不显著。控水试验结果表明:在水分胁迫条件下其蒸腾速率与其叶水势和土壤含水量的相关性最显著。     

Cu~(2+),Cu~(2+)/Vit C对亚油酸过氧化起动机理的ESR研究

本文直接用亚油酸为材料,利用ESR自旋捕集技术和紫外分光技术,分别检测了脂质过氧化反应过程中产生的中间自由基产物L·和共轭二烯结构,对Cu~(2+),Cu~(2+)/Vit C起动脂质过氧化反应的机理进行了研究。结果表明:Vit C自由基,Cu~(2+),Cu~+都不能起动纯的亚油酸的过氧化;Cu~(2+),Cu~+可以使含有部分过氧化成份的亚油酸继续氧化。     

青海省雏蝗属一新种记述：（直翅目：蝗总科：网翅蝗科）

在整理由青海省采得蝗虫标本时,发现1新种,记述如下。模式标本保存于山东大学生物系。青海雏蝗Chorthippus qinghaiensis,新种(图1—8) 雄:体小型。头部短于前胸背板。头顶平,侧缘明显隆起,顶锐角形。头侧窝狭长方形,长为宽的3.3倍。颜面向后倾斜,颜面隆起纵沟较浅,具刻点,侧缘在中眼处略狭。触角丝状,中段一节长为宽的1.4倍。复眼较小,卵圆形,纵径为横径的1.3倍,